: an Android - based application for biologists to plan RNA

RNA sequencing (RNA-seq) is a powerful technology that identifies novel transcripts (coding, non-coding and splice variants), understands transcript structures, and estimates gene/allele expression. Biologists face specific challenges while designing RNA-seq experiments. The nature of these challenges lies in determining the total number of sequenced reads and replicates required for detecting marginally differentially expressed transcripts, and in determining the adequate number of lanes to use in a sequencing flow cell. Despite previous attempts to address these challenges, easily accessible and biologist-friendly mobile applications do not exist. Thus, we developed , a mobile application for Android platforms, to aid biologists in correctly RNAtor designing their RNA-seq experiments. The recommendations from are RNAtor based on simulations and real data. Binay Panda ( ) Corresponding author: [email protected] : Formal Analysis, Methodology; : Software; : Formal Analysis, Writing – Review & Editing; : Author roles: Kane S Garg H Krishnan NM Singh A Formal Analysis, Methodology; : Conceptualization, Project Administration, Supervision, Visualization, Writing – Original Draft Panda B Preparation, Writing – Review & Editing Competing interests: No competing interests were disclosed. Kane S, Garg H, Krishnan NM How to cite this article: et al. : an Android-based application for biologists to plan RNA sequencing RNAtor 2017, :997 (doi: ) experiments [version 1; referees: 1 approved with reservations] F1000Research 6 10.12688/f1000research.11982.1 © 2017 Kane S . This is an open access article distributed under the terms of the , which Copyright: et al Creative Commons Attribution Licence permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Research presented in this article is funded by the Department of Electronics and Information Technology, Government of Grant information: India (Ref No: 18(4)/2010-E-Infra., 31-03-2010) and Department of IT, BT and ST, Government of Karnataka, India (Ref No: 3451-00-090-2-22). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 26 Jun 2017, :997 (doi: ) First published: 6 10.12688/f1000research.11982.1 1 1 1 1